3.9 Å structure of the yeast Mec1-Ddc2 complex, a homolog of human ATR-ATRIP

Journal: Science

Published: 2017-12-01

DOI: 10.1126/science.aan8414

Affiliations: 4

Authors: 8

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Research Highlight

Key DNA repair component imaged in exquisite detail

© lvcandy/Getty

© lvcandy/Getty

A near-atomic structural map of a master repair protein that is integral to fixing DNA damage could help drug developers to create new kinds of anti-cancer treatments.

Using electron microscopy, a team led by researchers at the University of Science and Technology of China determined the high-resolution structure of the yeast equivalent of the human ATR protein in tandem with its cell-signaling partner, ATRIP. These proteins form a complex that activates the cell’s built-in repair system at the first hint of DNA damage or errors in replication.

The detailed protein architecture — imaged to a resolution of 3.9 ångströms, about eight times the size of a single helium atom — revealed critical sites of regulation and activation that could serve as targets for future drug design efforts.

By inhibiting this complex, researchers hope to make cancer cells more sensitive to DNA-damaging chemotherapy or radiation.

Supported content

  1. Science 358, 1206–1209 (2017). doi: 10.1126/science.aan8414
Institutions FC
Hefei National Laboratory for Physical Sciences at the Microscale (HFNL), China 0.33
School of Life Sciences, USTC, China 0.33
MOA Key Laboratory of Agricultural and Environmental Microbiology, NAU, China 0.25
CAS Center for Excellence in Molecular Cell Science, SIBS CAS, China 0.08

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