Single-cell multi-omics sequencing of human early embryos

Journal: Nature Cell Biology

Published: 2018-06-18

DOI: 10.1038/s41556-018-0123-2

Affiliations: 11

Authors: 14

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Research Highlight

An epigenetic atlas of human embryos

© JUAN GARTNER/Science Photo Library/Getty

© JUAN GARTNER/Science Photo Library/Getty

As a human embryo develops from a fertilized egg into a complex cluster of cells ready for implantation in the womb, the chemical tags adorning its DNA undergo a series of organizational changes.

A Peking University–led team has now profiled the nature of this epigenetic complexity down to the single-cell level in sperm, eggs and six stages of pre-implantation embryos. The detailed map of modifications to DNA and its spool-like coiling structures provides a vast informative resource for future mechanistic studies of early human development.

The researchers showed that DNA−protein complexes known as chromatin were most loosely packaged — allowing genes and regulatory elements to be more accessible and active — in eggs and in single-cell zygotes. Chemical alterations then prompted the chromatin to tighten its coils over the first few cell divisions before loosening again (and staying loose) after about four days of development.

Supported content

  1. Nature Cell Biology 20, 847–858 (2018). doi: 10.1038/s41556-018-0123-2
Institutions FC
Beijing Advanced Innovation Center for Genomics (ICG), PKU, China 0.19
Department of Obstetrics and Gynecology, PUTH PKU, China 0.19
School of Life Sciences, PKU, China 0.19
MOE Key Laboratory of Assisted Reproduction, PUHSC PKU, China 0.10
Peking University Institute of Systems Biomedicine (PKUISBM), PUHSC PKU, China 0.09
MOE Key Laboratory of Cell Proliferation and Differentiation, PKU, China 0.09
MOE Key Laboratory of Birth Defects and Related Diseases of Women and Children, SCU, China 0.04
Department of Obstetrics and Gynecology, SCU, China 0.04
Center for Life Sciences (CLS), PKU, China 0.03
Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, PKU, China 0.03
Academy for Advanced Interdisciplinary Studies (AAIS), PKU, China 0.01

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