Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling

Journal: Nature Communications

Published: 2017-11-20

DOI: 10.1038/s41467-017-01560-x

Affiliations: 10

Authors: 14

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Research Highlight

Got milk? The development of mammary glands

© JGI/Jamie Grill/Blend Images/Getty

© JGI/Jamie Grill/Blend Images/Getty

Studying genes in breast cells before, during and after puberty has provided insights into how mammary glands develop. 

Female mammals produce milk in their mammary glands, or breasts, to feed offspring. The cells lining the gland wall, or epithelium, only become lactating ‘luminal cells’ in adulthood, but the genetic activity behind the timing of this change is unclear. 

A team including researchers from the University of Melbourne used single-cell RNA sequencing to compare genetic activity in thousands of mice breast epithelial cells pre-puberty, during puberty and in adulthood. They found that before puberty, all cells are in a similar, immature state, but on the cusp of puberty, cells begin to express genes that specify whether the basic cells become luminal cells. 

"This sort of technology can be applied to understanding which cells go awry in women at in increased risk for developing breast cancer” one of the authors, Jane Visvader, reports.

Supported content

  1. Nature Communications 8,1627 (2017) doi: 10.1038/s41467-017-01560-x
Institutions FC
ACRF Stem Cells and Cancer Division, WEHI, Australia 0.41
Department of Medical Biology, UniMelb, Australia 0.21
Division of Bioinformatics, WEHI, Australia 0.11
Australian Genome Research Facility (AGRF), Australia 0.07
Division of Systems Biology and Personalised Medicine, WEHI, Australia 0.07
Department of Biochemistry and Molecular Biology, UniMelb, Australia 0.04
School of Mathematics and Statistics, UniMelb, Australia 0.04
Melbourne Medical School, UniMelb, Australia 0.02
Royal Melbourne Hospital (RMH), Australia 0.02
Familial Cancer Centre, Australia 0.02

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