Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy

Journal: Nature Communications

Published: 2017-11-10

DOI: 10.1038/s41467-017-01466-8

Affiliations: 7

Authors: 7

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Research Highlight

And… cut! Gene-editing tool recorded in action

© KTSDESIGN/SCIENCE PHOTO LIBRARY/Getty

© KTSDESIGN/SCIENCE PHOTO LIBRARY/Getty

Real-time imaging of the popular gene-editing tool, CRISPR-Cas9, has revealed how molecules combine forces to cut DNA.

CRISPR-Cas9 uses an RNA molecule to guide the Cas9 protein to a specific section of DNA for Cas9 to cut so that genes can be added or removed. As imaging techniques can tamper with the target molecules this process has never before been observed in action. A team led by researchers from Kanazawa University used atomic force microscopy to record the molecules doing their job. The footage showed that Cas9 is usually a flexible, shape-shifting protein, but with the guide RNA attached, forms a fixed and stable structure. These newly built ‘molecular scissors’ head to their target on the DNA and change shape again to make the final cut.

This work confirms scientists’ theory of how CRISPR-Cas9 works and highlights the potential of atomic force microscopy in molecular imaging.

Supported content

  1. Nature Communications 8, 1430 (2017). doi: 10.1038/s41467-017-01466-8
Institutions FC
Department of Biological Sciences, UTokyo, Japan 0.43
Bio-AFM Frontier Research Center, KU, Japan 0.32
Precursory Research for Embryonic Science and Technology (PRESTO), JST, Japan 0.11
Institute for Frontier Science Initiative, KU, Japan 0.07
School of Mathematics and Physics, KU, Japan 0.04
Department of Physics, Nagoya University, Japan 0.04

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