Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites

Journal: Nature Biotechnology

Published: 2018-11-05

DOI: 10.1038/nbt.4283

Affiliations: 5

Authors: 9

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Research Highlight

A new way to interrogate the non-coding genome

© KEITH CHAMBERS/SCIENCE PHOTO LIBRARY/Getty

© KEITH CHAMBERS/SCIENCE PHOTO LIBRARY/Getty

A genome-wide screen for long RNAs that do not code for proteins has revealed many that are essential for cell growth.

A team from Peking University used CRISPR gene editing to target splice sites of long non-coding RNAs, thereby knocking out their function. By targeting around 11,000 RNAs in this way in three different human cell lines, they identified several hundred needed for the growth of one cell type or another.

Some of these RNAs were vital for the survival of all the cell lines tested — but there was a large degree of cell-type specificity in RNA function as well.

The findings offer a new platform for large-scale screening of non-coding genes. They also point to potential new drug targets for diseases of some of the cell types tested, including leukaemia.

Supported content

  1. Nature Biotechnology 36, 1203–1210 (2018). doi: 10.1038/nbt.4283
Institutions FC
Biomedical Pioneering Innovation Center (BIOPIC), PKU, China 0.23
Beijing Advanced Innovation Center for Genomics (ICG), PKU, China 0.23
Center for Life Sciences (CLS), PKU, China 0.23
Genome Editing Research Center, PKU, China 0.23
Academy for Advanced Interdisciplinary Studies (AAIS), PKU, China 0.09

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