Reconstitution in vitro of the entire cycle of the mouse female germ line

Journal: Nature

Published: 2016-10-17

DOI: 10.1038/nature20104

Affiliations: 9

Authors: 11

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Research Highlight

What came first, the mouse or the egg?

© ICHIRO/DigitalVision/Getty

© ICHIRO/DigitalVision/Getty

A team led by researchers at Kyushu University produced functional mouse eggs and stem cells entirely in a dish thanks to an innovative cell culture system. The findings open up possibilities in the fertility and regenerative medicine fields.

The researchers took either undifferentiated stem cells from mouse embryos or stem cells reprogrammed from adult cells, and generated immature eggs called ‘oocytes’. They were then able to grow and fertilize these oocytes while they were still in a dish. When transferred to mice wombs, a small proportion (3.5 per cent) of these embryos developed into healthy fertile animals, which shows that some of the eggs were functional. The researchers also isolated stem cells from the fertilized eggs and turned them into oocytes – thus achieving a full, self-sustaining cell culture cycle.

Although it is early days, some researchers hope that these findings will help researchers working in human fertility.

Supported content

  1. Nature 539,299–303 (2016). doi: 10.1038/nature20104
Institutions FC WFC
Department of Stem Cell Biology and Medicine, Kyushu University, Japan 0.68 0.68
Faculty of Applied Biosciences, Tokyo University of Agriculture, Japan 0.09 0.09
NARO Institute of Livestock and Grassland Science (NILGS), Japan 0.09 0.09
School of Medicine / Graduate School of Medical Sciences / Faculty of Medical Sciences, Kyushu University, Japan 0.05 0.05
Department of Anatomy and Cell Biology, Kyoto University, Japan 0.03 0.03
Center for iPS Cell Research and Application (CiRA), Kyoto University, Japan 0.03 0.03
WPI Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Japan 0.03 0.03

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