Single Cell Level Quantification of Nanoparticle-Cell Interactions Using Mass Cytometry

Journal: Analytical Chemistry

Published: 2017-08-15

DOI: 10.1021/acs.analchem.7b01006

Affiliations: 8

Authors: 6

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Research Highlight

Sensing silver in single cells

© KATERYNA KON/SCIENCE PHOTO LIBRARY/Getty

© KATERYNA KON/SCIENCE PHOTO LIBRARY/Getty

Probing the properties of single cells can reveal the amount of metal particles they have absorbed.

Metal nanoparticles, typically particles less than 100 nanometres wide, can make their way into  human cells with potentially harmful effects. Measuring the concentration of these nanoparticles inside single cells remains a challenge. A team including researchers from the University of South Australia used mass cytometry — a technique for simultaneously measuring many properties of individual cells — to ascertain the silver content inside human immune cells. The cells all absorbed different quantities of silver, revealing the importance of single cell analysis when studying how cells and nanoparticles interact.

This technique could reveal which nanotechnologies pose a risk to humans, and guide research into which nanoparticles could be used in medicine.

Supported content

  1. Analytical Chemistry 89, 8228-8232 (2017). doi: 10.1021/acs.analchem.7b01006
Institutions FC
Future Industries Institute, UniSA, Australia 0.25
Melbourne School of Engineering, UniMelb, Australia 0.17
Adelaide Medical School, Adelaide Uni, Australia 0.17
Women's and Children's Hospital (WCH), SA Health, Australia 0.17
Laboratory of Environmental Toxicology, NICPB, Estonia 0.08
Drug Delivery, Disposition and Dynamics (D4), Monash University, Australia 0.06
ANFF Melbourne Centre for Nanofabrication (MCN), Australia 0.06
CSIRO Manufacturing, Australia 0.06

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