An electrochemical method for detecting the biomarker 4-HPA by allosteric activation of Acinetobacter baumannii reductase C1 subunit
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A new electrochemical assay could help reveal signs of intestinal bacterial imbalance, cancer and other health problems.
The chemical 4-hydroxy-phenylacetate (4-HPA) is found in urine. It is a potential biomarker for imbalances in gut bacteria and for neural disorders and some cancers.
Now, a team led by researchers from Vidyasirimedhi Institute of Science and Technology in Thailand has developed a test to detect 4-HPA.
The team created a simple biosensor that exploits a bacterial enzyme that works together with 4-HPA to modulate a key energy metabolism pathway, with hydrogen peroxide produced as a by-product. In the presence of 4-HPA, more hydrogen peroxide is thus produced, which can be easily measured via an electrochemical readout.
The researchers next plan to clinically validate it on urine samples from patients.
- Journal of Biological Chemistry 296, 100467 (2021). doi: 10.1016/j.jbc.2021.100467
|Vidyasirimedhi Institute of Science and Technology (VISTEC), Thailand||0.50|
|Suranaree University of Technology (SUT), Thailand||0.17|
|Chulalongkorn University (CU), Thailand||0.17|
|Kindai University, Japan||0.17|