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Connecting proteins with calixarene molecular glue
Calixarenes are large molecules with atoms arranged in a ring with a puckered structure. A team from Flinders University in Australia and colleagues has shown that the unusual shape of calixarenes can mimic protein binding sites and fix elusive biomolecules into well-defined crystal lattices.
researchers deduced that calixarene discs loaded with phosphorous-oxygen ions attach
to a single binding site on the protein cytochrome c. They then stack two
proteins back-to-back to form a ‘dimer’ complex. These small nanostructures drive
crystallization of an ordered complex that locks the proteins into place, and
keeps them at distances from each other — an ideal situation for x-ray-based structure
analysis of this and possibly other biomolecules.
- Angewandte Chemie International Edition 56, 5517–5521 (2017). doi: 10.1002/anie.201701500
|School of Chemistry, NUI Galway, Ireland||0.75|
|Institute for Nanoscale Science and Technology, Flinders University, Australia||0.25|