Nanoparticle-Based Fluoroionophore for Analysis of Potassium Ion Dynamics in 3D Tissue Models and In Vivo

Journal: Advanced Functional Materials

Published: 2018-02-28

DOI: 10.1002/adfm.201704598

Affiliations: 8

Authors: 14

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Research Highlight

The brighter side of encapsulation

©JUAN GAERTNER/SCIENCE PHOTO LIBRARY/Getty

©JUAN GAERTNER/SCIENCE PHOTO LIBRARY/Getty

Polymer-encapsulated fluorescent dyes could help us understand ion fluxes that regulate essential processes in the body, such as cell growth and cross-membrane transport.

A University College Cork-led team has developed a versatile potassium-sensitive fluorescent probe that penetrates and stains a broad range of tissues, including neurons, cancer cells, and organ models, with unexpectedly high efficiency. To generate the probes, the researchers encased ion-selective, red-light-emitting dye molecules in tiny polymer particles, enhancing the fluorescence intensity of the dye three-to five-fold inside the target cells. 

Live cell assays demonstrated that the probes can be used to monitor intracellular potassium fluxes in the brain, gut, and other tissues. Brain imaging experiments revealed that the probes can facilitate the visualization of epileptic seizures, which can be useful for developing better therapies. This approach may also provide bright, selective, and photo-stable probes for high-resolution cell imaging techniques, such as confocal and two-photon microscopies.

Supported content

  1. Advanced Functional Materials 28, 1704598 (2018). doi: 10.1002/adfm.201704598
Institutions FC
Institute of Analytical Chemistry and Food Chemistry, TU Graz, Austria 0.21
Department of Anatomy and Neurobiology, UMB, United States of America (USA) 0.14
Fischell Department of Bioengineering, UMCP, United States of America (USA) 0.14
Laboratory for Biomimetic Membranes and Textiles, EMPA, Switzerland 0.14
School of Biochemistry and Cell Biology, UCC, Ireland 0.13
Analytical and Biological Chemistry Research Facility (ABCRF), UCC, Ireland 0.13
Department of Anatomy and Neuroscience, UCC, Ireland 0.07
University College Cork (UCC), Ireland 0.02

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